Fig. 5

Coomassie-blue stained gel of normal liver 2-D resolved proteins. Proteins immunoreactive with more than 30 % of the 13 CC sera are indicated by arrows and listed in Additional file 2: Table S2. Liver arginase 1 corresponding to arrows NH1 and NH2 and glyceraldehyde-3-phosphate dehydrogenase (NH6, NH11) were recognized by 54 % of 13 CC sera, 3 ketoacyl-COA thiolase corresponded to arrows NH8 and NH9 (46 % of CC sera). Aconitate hydratase (NH16), bifunctional ATP-dependant dihydroxyacetone kinase (NH13), electron transfer-flavoprotein α (NH15), estradiol 17-β-dehydrogenase 8 (NH3), fructose-1.6 biphosphatase 1 and fructose-biphosphate aldolase B (both identified in the same spot NH10), S-methyl-5′ thioadenosine phosphorylase (NH7), were each recognized by 38 % of the CC sera. Proteins recognized by 31 % of the sera were: acetyl CoA acetyl transferase mitochondrial (NH18), aldhehyde dehydrogenase (NH14), carbonic anhydrase 1 (NH5), Δ(3,5) Δ(2,4) dienoyl Coa isomerase (NH4), Δ-1-pyrroline-5-carboxylate dehydrogenase (NH12) and prelamine A/C (NH17)