Fig. 4

IL-33 promoted anti-RBC antibody production. a PBMCs from AIHA patients (n = 6) were stimulated with anti-IgM, CD40L in the presence of indicated dose of IL-33 for 6d, and then assayed for IgG antibody production. One dot represented the result from one patient; b PBMCs from AIHA patients (n = 5) were stimulated with anti-IgM, CD40L plus IL-33 (20 ng/ml) in the presence of IL-33 neutralizing antibody or control antibody (2 μg/ml) for 6d, and detected for IgG antibody; c–e PBMCs from AIHA patients (n = 4) were stimulated with IL-33 (20 ng/ml) for 72 h, followed by detection for production of IL-4, IL-6 and IL-13 in supernatants