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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: MicroRNA regulation of unfolded protein response transcription factor XBP1 in the progression of cardiac hypertrophy and heart failure in vivo

Fig. 5

miR-214 and miR-30* reduce the target of XBP1 expression in cardiomyocytes. a Western blot analysis of XBP1 in H9C2 (2-1) cells co-transfected with miR-30a* inhibitors (antimiR-30a*) and miR-214 mimics. b Western blot analysis of XBP1, VEGF and EDEM in H9C2 (2-1) cells transfected with or without miR-30a* and miR-214 mimics or miRNA negative control oligonucleotides (100 nM). c Real-time PCR analysis of VEGF and EDEM mRNA in H9C2 (2-1) cells transfected with or without miR-30a* and miR-214 mimics or miRNA negative control oligonucleotides (100 nM). d Real-time PCR analysis of VEGF expression in untreated and TG-treated H9C2 (2-1) cells transfected with or without miR-30a* or miR-214 mimics or miR-NC (100 nM). n = 3. *P < 0.05 compared with control

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