Fig. 3

XBP1 is experimentally validated as a direct target of miR-30a*. a Expression of the miR-214 and miR-30* family in normal mice heart tissue. n = 6. b a schematic diagram of the reporter constructs showing the entire XBP1 3′ UTR sequence and the sequence of the miR-30* binding sites within the human XBP1 3′ UTR and MUT 3′ UTR. c Western blot of XBP1 in H9C2 (2-1) cells 48 h after transfection of miR-30* mimics or miRNA negative control (miR-NC) oligonucleotides. d Luciferase activity 24 h after transfection of the pMIR-XBP1-3′ UTR reporter or pMIR-XBP1-3′ UTR mut reporter and miRNAs. e Western blot of XBP1 in H9C2 (2-1) cells 48 h after transfection of miR30a* inhibitors (antimiR-30*) or miRNA inhibitors negative control (antimiR-NC) oligonucleotides (100 nM). *P < 0.05 compared with miR-NC