Fig. 1
From: Early outgrowth cells versus endothelial colony forming cells functions in platelet aggregation
Characterization of PBMC-derived EOCs and ECFCs. a Representative optical microscopy images of EOCs and ECFCs taken at 10× magnification using an inverted light microscope. EOCs display a heterogeneous population of round and elongated cells on fibronectin following 7 days of culture. ECFCs show a homogeneous population forming a cobblestone-like monolayer on collagen following 21 days of culture. b Representative confocal microscopy images showing EOCs and ECFCs triple staining for DiI-Ac-LDL uptake (red), Ulex-lectin binding (green) and TO-PRO-3 nuclear staining (blue) taken at 63× magnification. c Representative optical microscopy images showing the tube-like structure formation potential of EOCs and ECFCs compared to HUVECs on a Matrigel surface taken at 10× magnification using an inverted microscope