Fig. 4
MSCs display characteristic immunophenotype and trilineage differentiation potential independent of medium type. Flow cytometric analysis of UC-MSCs, group 1 (a), UC-MSCs, group 2 (b) and BM-MSCs, group3 (c) revealed comparable immunophenotype profiles: CD73+/90+/105+/NG-2+ and CD14−/19−/31−/34−/45−/144−/271−/HLA-DR−. Histogram plots show representative results of UC-MSCs, group 1 consistently propagated in medium A (+fib/+hep), UC-MSCs, group 2 in medium C (−fib/+hep) and BM-MSCs, group 3 in medium A. d–f UC- and BM-MSCs display a similar in vitro trilineage potential for adipogenic (Sudan III staining), osteogenic (Alizarin Red staining) and chondrogenic (Alcian Blue staining, Nuclear Fast Red (NFR) staining) differentiation compared to controls. Total magnification: 200Ă— adipogenic differentiation, 100Ă— osteogenic differentiation, 40Ă— and 100Ă— chondrogenic differentiation