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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Mechanical fibrinogen-depletion supports heparin-free mesenchymal stem cell propagation in human platelet lysate

Fig. 4

MSCs display characteristic immunophenotype and trilineage differentiation potential independent of medium type. Flow cytometric analysis of UC-MSCs, group 1 (a), UC-MSCs, group 2 (b) and BM-MSCs, group3 (c) revealed comparable immunophenotype profiles: CD73+/90+/105+/NG-2+ and CD14/19/31/34/45/144/271/HLA-DR. Histogram plots show representative results of UC-MSCs, group 1 consistently propagated in medium A (+fib/+hep), UC-MSCs, group 2 in medium C (−fib/+hep) and BM-MSCs, group 3 in medium A. df UC- and BM-MSCs display a similar in vitro trilineage potential for adipogenic (Sudan III staining), osteogenic (Alizarin Red staining) and chondrogenic (Alcian Blue staining, Nuclear Fast Red (NFR) staining) differentiation compared to controls. Total magnification: 200× adipogenic differentiation, 100× osteogenic differentiation, 40× and 100× chondrogenic differentiation

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