Skip to main content
Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Mechanical fibrinogen-depletion supports heparin-free mesenchymal stem cell propagation in human platelet lysate

Fig. 2

Experimental workflow: Isolation and characterization of umbilical cord (UC-) and bone marrow (BM-) MSCs. UC-MSCs group 1 (left) and BM-MSCs, group3 (right) were isolated using medium condition A exclusively. UC-MSCs group 2 (middle) were isolated in all three medium conditions (A, white; B, grey and C, black). After isolation and expansion, all MSC types tested by flow cytometric analysis and differentiation assays. Proliferation and colony forming unit assays (CFU) were performed independently in triplicates over a minimum of seven population doublings (UC-MSCs: passages 1–3, BM-MSCs: passages 2–4) examining all three medium types (A, B and C)

Back to article page