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Fig. 8 | Journal of Translational Medicine

Fig. 8

From: Application of Nanotrap technology for high sensitivity measurement of urinary outer surface protein A carboxyl-terminus domain in early stage Lyme borreliosis

Fig. 8

Viral and Lyme disease co-infection pathogens were devoid of immunoreactivity with the mAb clone 0551. Infection with these common non-Lyme pathogens do not generate a false positive for Borrelia in the present Nanotrap test. a Herpes simplex 1 and Epstein Barr viral lysates were mixed with urine in presence and absence of Bb antigen. Samples were processed with Nanotrap particles and analyzed with western blot. Lane 1 ladder, lane 2 Bb antigen 1 ng; lanes 3–7 eluates of Nanotrap particles incubated with urine without Bb antigens (lane 3), 25 pg/mL Bb antigen in urine 40 mL (lane 4), HSV-1 lysate (1 μg in 40 mL of urine) (lane 5), EBV lysate (1 μg in 40 mL of urine) (lane 6), HSV-1 lysate (1 μg in 40 mL of urine) plus EBV lysate (1 μg in 40 mL of urine) plus Bb antigen (1 ng in 40 mL of urine) (lane 7). (B) Hepatitis C and Cytomegalovirus viral lysates were mixed with human urine in presence and absence of Bb antigen. Lane 1: ladder, lane 2 Bb antigen 1 ng; lanes 37: eluates of Nanotrap particles incubated with urine without Bb antigen (lane 3), 25 pg/mL Bb antigen in urine 40 mL (lane 4), HCV lysate (1 μg in 40 mL of urine) (lane 5), CMV lysate (1 μg in 40 mL of urine) (lane 6), HCV lysate (1 μg in 40 mL of urine) plus CMV lysate (1 μg in 40 mL of urine) plus Bb antigen (1 ng in 40 mL of urine) (lane 7). c Bartonella henselae and Babesia microti lysates were spiked in human urine in presence and absence of Bb antigen. Lane 1: ladder, lane 2: Bb antigen 1 ng; lanes 3–9: eluates of Nanotrap particles incubated with urine without Bb antigen (lane 3), 25 pg/mL Bb antigen in urine 40 mL (lane 4), Bartonella lysate (1 ng in 40 mL of urine) (lane 5), Babesia lysate (10 ng in 40 mL of urine) (lane 6), red blood cells (10 ng in 40 mL of urine) (lane 7), Bartonella lysate (10 ng in 40 mL of urine) plus Babesia lysate (10 ng in 40 mL of urine) plus Bb antigen (1 ng in 40 mL of urine) (lane 8), 40 mL of urine of a patients with Bartonella positive and Borrelia negative serology at the time of urine collection (lane 9)

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