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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Application of Nanotrap technology for high sensitivity measurement of urinary outer surface protein A carboxyl-terminus domain in early stage Lyme borreliosis

Fig. 2

Mapping the OspA antigen epitope. Bb Lyme antigen was partially digested with pepsin and the protease derived fragments were split into two aliquots. One aliquot was analyzed by western blotting with the anti-OspA mAb clone 0551. In parallel, the second aliquot of pepsin fragments was analyzed by SDS PAGE and silver stained. The band in the SDS PAGE mirroring the smallest peptide fragment recognized by the mAb was cut out and processed for mass spectrometry analysis. MS/MS spectra of the smallest peptides that reacted with the mAb are shown

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