Fig. 3

TGF-β induced EMT in murine and human TECs. TGF-β stimulation was performed in murine TCMK-1 and human HK-2 cells. After 72 h, the levels of E-cadherin, vimentin and α-SMA in a TCMK-1 and b human HK-2 cells with varied levels of TGF-β (0, 2.5, 5 and 10 ng/ml) were determined by western blotting. c, d Quantitative results presented as the mean ± SD of the optical density of each band, n = 6. *p < 0.05 compared with the TGF-β control group (0 ng/ml)