Fig. 8

Elution profile of GGT activity from whole homogenate of three selected plaque samples (a, b, c). Elution profile was obtained by molecular exclusion chromatography, and GGT activity was detected by an online post-column reaction in the presence of a fluorescent substrate. A major peak of activity eluting at 12.9 ml and corresponding to the b-GGT fraction is detectable. d Western blot analysis of GGT heavy chain in samples of isolated plaque b-GGT and other biological samples. Lanes 1–3 b-GGT from plaques #1–3, respectively; lane 4 monocytes; lane 5 HMEC-1 endothelial cells; lane 6 lymphocytes; lane 7 platelets