Fig. 7

GGT expression in atherosclerotic plaques. A representative thin cap fibroatheroma without ulceration is shown. Masson’s trichrome staining (a; blue collagen fibers; red cytoplasm; black nuclei); immunohistochemistry for GGT protein (b) and macrophage-associated antigen CD68 (c). GGT and CD68 were localized by immunoperoxidase reaction with 3-diaminobenzidine as substrate, yielding a reddish-brown color. Immunoreactivities are mainly localized in the necrotic core (asterisk) extending just beneath the endothelial lining of the plaque (arrow). Sections were couterstained with haematoxylin. Original magnification: 10x