Fig. 4

GGT release by activated macrophages. Monocytes isolated from fresh buffy coats were incubated for 6 days in complete medium in the presence of GM-CSF (50 ng/ml) or M-CSF (50 ng/ml) in order to induce the differentiation into M1-like or M2-like macrophages, then they were incubated in the presence of TNFα/IL-1β (both 10 ng/ml) for additional 24 h. GGT activity was measured in the 10,000×g centrifuged supernatants (a, b) and in cellular homogenates (c, d). Results are mean ± SD of eight separate determinations. Data are expressed as mU/mg of cellular proteins and were analyzed by Student’s t test for paired data; *p < 0.05 as compared with the corresponding untreated control