Fig. 3

Effects of GM-CSF and M-CSF exposure on cellular GGT expression. Monocytes isolated from fresh buffy coats were incubated for 6 days in complete medium in the presence of GM-CSF (50 ng/ml) or M-CSF (50 ng/ml) in order to induce the differentiation into M1-like or M2-like macrophages, respectively. a Data are ratioes between GGT activities determined after differentiation and activities present in initial monocytes. The results of ten separate paired incubations are shown. Solid lines connect data pairs obtained with the same starting monocytes preparation. Statistical analysis was performed by Student’s t test for paired data; *p = 0.0016. b Representative western blot analysis of GGT expression. Lane 1 monocytes; lane 2 M1-like macrophages; lane 3 M2-like macrophages