Fig. 4
Effects of cofilin1 depletion on stress fibre formation. a AngII induced stress fibre formation in HK2. Confluent HK2 cells were untreated (left) or challenged for 12 h with AngII (right). The cells were then fixed, permeabilized, and stained with rhodamine-labeled phalloidin to visualize the actin stress fibres by fluorescence microscopy. Original magnification ×200, scale bar represents 100 μm. b, c Effect of lentiviruses transfection in HK2. The prepared HK2 cells were transfected with lentiviruses containing cofilin1 shRNA (Lv-cofilin1-shRNA) and nonspecific control lentiviruses (Lv-control-shRNA) at MOI of 50. b The infection efficiency was measured after 72 h using a fluorescence microscope via observing the expression of green fluorescent protein. Original magnification ×200, scale bar represents 100 μm. c Total transfected cell lysates were prepared and immunoblotted with an anti-cofilin1 antibody. d–f Cofilin-1 depletion promoted stress fibre formation. After passage, the cells transfected lentiviruses were challenged for 12 h with AngII (10−6mol L−1). d Stress fibre was visualized by fluorescence microscope. Original magnification ×400, scale bar represents 50 μm. e, f Representative Western blotting for F-actin, Cells in group a, c were only transfected with Lv-control-shRNA and Lv-cofilin1-shRNA respectively. Cells in group b, d were transfected with Lv-control-shRNA and Lv-cofilin1-shRNA respectively, then stimulated with AngII after transfection, *P < 0.05, **P < 0.01 vs. group a; #P < 0.05 vs. group b; ΔΔP < 0.01 vs. group c