The tumor area occupied by Tbet+ cells in deeply invading cervical cancer predicts clinical outcome

Table 2 Differences in immune cell infiltration between groups

	No		Yes		p value^a
	Mean	SEM	Mean	SEM	p value^a
HPV-specific T cells in blood^b
Area of CD45 cells (%)	9.76	2.51	15.47	2.93	0.203
Total cell pixel count	5378	619	5678	377	0.667
Tbet+ cells pixel count	244	72.0	434	185	0.485
Foxp3+ cells pixel count	125	25.9	142	24.2	0.648
LN metastases present
Area of CD45 cells (%)^c	20	3.3	14	2.0	0.119
Total cell pixel count^d	5686	339	5655	183	0.942
Tbet+ cells pixel count^d	405	118	174	42	0.097
Foxp3+ cells pixel count^d	163	27.5	124	16.8	0.217
Tumor size ≥40 mm
Area of CD45 cells (%)	20	3.4	16	2.5	0.346
Total cell pixel count	5806	440	5590	189	0.656
Tbet+ cells pixel count	488	162	189	40.6	0.087
Foxp3+ cells pixel count	170	27.4	124	18.7	0.152
Area CD45 cells (%) ≥ median
Total cell pixel count	5460	264	5919	279	0.239
Tbet+ cells pixel count	114	27.9	328	55.0	0.002
Foxp3+ cells pixel count	80.6	12.5	196	25.9	0.000

^aDifferences in means were assessed by independent sample t test, p < 0.05 is significant
^bWithin the current study group 17 patients have been tested for the presence of circulating HPV-specific T cells, 10 of them were negative [4]
^cThe area occupied by CD45 cells within the tumor was calculated as a percentage
^dThe pixel count of cells per high power field (20×) was analysed in 4 different fields

ISSN: 1479-5876