Fig. 1
From: The tumor area occupied by Tbet+ cells in deeply invading cervical cancer predicts clinical outcome
Schematic representation of the automated quantitative method used. A representative image of a scanned slide (a). After deconvoluting the haematoxylin (b) and DAB (c) colors, the total tissue area was selected using a 100 % threshold for the haematoxylin staining (d). Then the DAB positive area was determined (e). The number of pixels in image e was divided by the number of pixels in image d and multiplied by 100 to calculate the tumor percentage expressing DAB