Fig. 4

Silence of Fra-1 impaired cell motility in wound healing assay without significantly affecting cell viability. a The efficiency of siRNA was confirmed by real-time PCR. The relative Fra-1 mRNA levels were determined by RT-PCR in siRNA transfected cells (n = 3; *P < 0.05). b The knock-down of Fra-1 via RNAi technique reduced the expression of Fra-1 at protein level, while GAPDH used as a loading control (n = 3; *P < 0.05). c Transfection of siFra-1 had no apparent effect on prostate cancer cell viability, concentration of the reagent and time course of the cell proliferation (24, 48 and 72 h after transfection) were provided. d Wound healing assays with siFra-1 in DU145 and PC3 cells were performed at 24 and 48 h, the wound area was measured by Image J software (*P < 0.05)