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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: MicroRNA-195-5p, a new regulator of Fra-1, suppresses the migration and invasion of prostate cancer cells

Fig. 3

Transfection with miR-195 impaired the motility of prostate cancer cells and Fra-1 is a direct target of miR-195. a, c DU145 and PC3 cells were transfected with NC, miR-195 mimics and cell migration and invasion were assessed after 48 h incubation by transwell assay. Representative micrographs of the cell migration and invasion assay (left) and quantification (right) from three independent experiments were presented at ×100 (*P < 0.05). b, e The Fra-1 expression level was significantly reduced following treatment with miR-195 mimics by RT-PCR and western blot in DU145 and PC3 cells. d Representative western blotting results of motility-related proteins in miR-195-transfected DU145 and PC3 cells, band intensity quantification was made by Image J software, while GAPDH used as a loading control (n = 3; *P < 0.05). f A schematic of the computational predicted seed region in the 3′-UTR of Fra-1 (conserved in human, rhesus, dog, rat, mouse) was shown, while mutations on the “seed” sequences were designed as below. HEK293T cells were co-transfected with 50 nM of either miR-195 or NC and 200 ng pmirGLO Dual-Luciferase miRNA Target Expression Vector comprising Wt or Mut 3′-UTR of Fra-1. miR-195 significantly suppressed the firefly luciferase activity of construct with Wt 3′-UTR of Fra-1 (*P < 0.05)

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