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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: YangZheng XiaoJi exerts anti-tumour growth effects by antagonising the effects of HGF and its receptor, cMET, in human lung cancer cells

Fig. 3

The effect of YangZheng XiaoJi and cMET kinase inhibitors, PHA665752 and SU11274 on HGF-induced cell migration (A, B, C, D) and invasion (a, b, c, d), on both SKMES1 (top panel) and A549 (bottom panel) cells. Migration assay A both PHA665752 (10 nM) and SU11274 (20 nM) had a significant effect on HGF-induced migration of SKMES1 cells. HGF concentration shown here is 50 ng/ml. *p < 0.01 cells with HGF/PHA665752 combination vs cells with control medium only; **p < 0.05 cells with HGF/SU11274 combination vs cells with HGF only. Ɵp = 0.059 cells with HGF alone vs cells with control medium. B The effect of the combination between DME25 and HGF inhibitors on SKMES1 cells. *p < 0.05 cells with HGF/SU11274 (or HGF/PHA665752) combination vs cells with HGF only; **p < 0.05 cells with HGF/SU11274/DME25 (or HGF/PHA665752/DME25) combination vs cells with HGF only; #p < 0.05 cells with HGF/PHA665752 combination vs cells with control medium only; Ɵp = 0.059 cells with HGF alone vs cells with control medium. C effect of HGF and HGF receptor inhibitors on the migration of A549 cells. *p < 0.01 cells with HGF/SU11274 (or HGF/PHA665752) combination vs cells with HGF only; #p < 0.05 respective cells vs cells with control medium only. D effect of HGF, and the combination of DME25 and HGF receptor inhibitors on the migration of A549 cells. *p < 0.01 cells with HGF/SU11274 (or HGF/PHA665752) combination vs cells with HGF only; **p < 0.05 cells with HGF/SU11274/DME25 (or HGF/PHA665752/DME25, or HGF/DME25) combination vs cells with HGF only; #p < 0.05 respective cells vs cells with control medium only. Invasion assay a, b DME25 significantly potentiated the effect of HGF receptor inhibitors, PHA665752 (a) and SU121274 (b), on HGF-induced invasion of SKMES1 cells. The effects were almost identical on both compounds. *p < 0.01 treatment plus DME25 vs respective treatment without DME25 control; **p < 0.05 treatment plus DME25 vs respective treatment without DME25 control; #p < 0.05 respective cells vs cells with control medium only. c, d Effects of DME25 on HGF and HGF receptor inhibitors, PHA665752 (c) and SU121274 (d), on HGF-induced invasion of A549 cells. *p < 0.01 treatment plus DME25 vs respective treatment without DME25 control; **p < 0.05 treatment plus DME25 vs respective treatment without DME25 control; # p < 0.05 respective cells vs cells with control medium only. The concentrations use for the test agents were: PHA665752—10 nM, SU11274—20 nM, HGF—50 ng/ml and DME25—1:1000 dilution

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