Fig. 3From: YangZheng XiaoJi exerts anti-tumour growth effects by antagonising the effects of HGF and its receptor, cMET, in human lung cancer cellsThe effect of YangZheng XiaoJi and cMET kinase inhibitors, PHA665752 and SU11274 on HGF-induced cell migration (A, B, C, D) and invasion (a, b, c, d), on both SKMES1 (top panel) and A549 (bottom panel) cells. Migration assay A both PHA665752 (10 nM) and SU11274 (20 nM) had a significant effect on HGF-induced migration of SKMES1 cells. HGF concentration shown here is 50 ng/ml. *p < 0.01 cells with HGF/PHA665752 combination vs cells with control medium only; **p < 0.05 cells with HGF/SU11274 combination vs cells with HGF only. Ɵp = 0.059 cells with HGF alone vs cells with control medium. B The effect of the combination between DME25 and HGF inhibitors on SKMES1 cells. *p < 0.05 cells with HGF/SU11274 (or HGF/PHA665752) combination vs cells with HGF only; **p < 0.05 cells with HGF/SU11274/DME25 (or HGF/PHA665752/DME25) combination vs cells with HGF only; #p < 0.05 cells with HGF/PHA665752 combination vs cells with control medium only; Ɵp = 0.059 cells with HGF alone vs cells with control medium. C effect of HGF and HGF receptor inhibitors on the migration of A549 cells. *p < 0.01 cells with HGF/SU11274 (or HGF/PHA665752) combination vs cells with HGF only; #p < 0.05 respective cells vs cells with control medium only. D effect of HGF, and the combination of DME25 and HGF receptor inhibitors on the migration of A549 cells. *p < 0.01 cells with HGF/SU11274 (or HGF/PHA665752) combination vs cells with HGF only; **p < 0.05 cells with HGF/SU11274/DME25 (or HGF/PHA665752/DME25, or HGF/DME25) combination vs cells with HGF only; #p < 0.05 respective cells vs cells with control medium only. Invasion assay a, b DME25 significantly potentiated the effect of HGF receptor inhibitors, PHA665752 (a) and SU121274 (b), on HGF-induced invasion of SKMES1 cells. The effects were almost identical on both compounds. *p < 0.01 treatment plus DME25 vs respective treatment without DME25 control; **p < 0.05 treatment plus DME25 vs respective treatment without DME25 control; #p < 0.05 respective cells vs cells with control medium only. c, d Effects of DME25 on HGF and HGF receptor inhibitors, PHA665752 (c) and SU121274 (d), on HGF-induced invasion of A549 cells. *p < 0.01 treatment plus DME25 vs respective treatment without DME25 control; **p < 0.05 treatment plus DME25 vs respective treatment without DME25 control; # p < 0.05 respective cells vs cells with control medium only. The concentrations use for the test agents were: PHA665752—10 nM, SU11274—20 nM, HGF—50 ng/ml and DME25—1:1000 dilutionBack to article page