Fig. 1

Successful gene transfer of Foxp3 in MSC and Foxp3-MSC engraftment in liver allograft. a MSC were infected with control lentivirus (FUGW, Green) and GFP-Foxp3 lentivirus (Foxp3, Green), Nucleus were stained by DAPI (Blue). MSC were efficiently transduced with >95 % GFP-positive cells. b The expression of Foxp3 was detected by western blot in Foxp3-MSC and FUGW-MSC lysis at 96 h after infection. The production of Foxp3-GFP fusion protein was confirmed in Foxp3-MSC lysis compared with FUGW-MSC lysis. c Liver allograft sections were analyzed by fluorescence microscopy for the presence of GFP-Foxp3-MSC, substantial numbers of GFP-Foxp3-MSC liver graft were observed in liver allograft (upper panel, original magnification ×400; lower panel, original magnification ×100)