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Table 1 Ion transitions and instrumental parameters for their LC–MS/MS quantification in MRM mode

From: Mother root of Aconitum carmichaelii Debeaux exerts antinociceptive effect in Complete Freund’s Adjuvant-induced mice: roles of dynorphin/kappa-opioid system and transient receptor potential vanilloid type-1 ion channel

Sample

Ion mode

MRM

CV

CE

LOQ

LOD

Benzoylmesaconine

+

590.33 > 77.02

52

78

2.1

0.73

Aconitine

+

646.39 > 586.34

50

34

1.9

0.6

Mesantine

+

632.33 > 105.04

42

56

2.2

0.9

Benzoylhypacoitine

+

574.32 > 542.35

46

34

1.8

0.6

Benzoylaconitine

+

604.41 > 105.04

58

54

4.3

1.7

Hypaconitine

+

616.36 > 556.31

42

32

1.9

0.61

Reserpine(is)

+

609.33 > 174.07

40

42

  1. IS internal standard, CE collision energy (eV), CV cone voltage (V), MRM specific mass transition, LOQ limit of quantification (ng/ml), LOD limit of detection (ng/ml)