Fig. 3

Inhibitory effect of SAR317461 on the proliferation of GBM lines. GSCs and established cells from established GBM lines were seeded into each well of a 96-well micro-culture plate and SAR317461 (40–0.075 μM) was added. Each point in every curve shows the mean for three samples and the bars are standard error of the mean. The standard deviation of IC₅₀ values for 7 tumorsphere lines treated with SAR317461 IC₅₀ value is approximately 8.1 with a mean of 4.88 (4.88 ± 8.1). Hence the IC₅₀ of 25 for SK892, which does not express pSTAT3, lies more than 2 standard deviations outside this interval. The IC₅₀s for U87, A172 and U251 are 7-8 μM. a (SK1035, SK987 and SK892), b (GBM4 and GBM8), c (SK262, SK429) are IC₅₀ curves for patient derived cell lines. d IC₅₀ curve of the U87, A172 and U251 immortalized GBM cell lines. e Shows representative images of GBM8 cells treated with 10 µM SAR317461 and control solution (DMSO).