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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: In vitro response pattern of monocytes after tmTNF reverse signaling predicts response to anti-TNF therapy in rheumatoid arthritis

Fig. 1

Monocytic surface expression of TNFR1 and CD54 are linked to anti-TNF treatment response. a Scatterplot showing the correlation of CD54 expression on ex vivo separated RA-monocytes with the decrease in the patients’ DAS28 (ΔDAS28) after 12 weeks (n = 18). Receptor expression is given as mean fluorescence intensity as determined by FACS. b Bar graph depicts mean and SEM of CD54 expression (mean fluorescence) in the study cohort on monocytes in patients with a good EULAR response (mean 62.1 ± 8.4) and non-responders (mean 83.48 ± 3.99), respectively. c Cell surface expression of CD54 was determined by flow cytometry on freshly isolated monocytes. Shown are representative histograms from one patient with a good response (light grey curve) and from a non-responder (dark grey curve) in comparison to the isotype controls (white curve; dark grey line for non-responder, light grey line for good responder). d, e Scatterplot showing the correlation between TNFR1 expression determined ex vivo on RA monocytes and the decrease in DAS28 (ΔDAS28) after 12 weeks (n = 17) (d) and 24 weeks (n = 18) (e). TNFR1 expression was determined by mean fluorescence intensity. f Cell surface expression of TNFR1 on freshly isolated monocytes. Shown are representative histograms from one patient with a good response (dark grey filled curve) and from a non-responder (light grey filled curve) in comparison to the isotype controls (dark grey line for good responder, light grey line for non-responder). Non-responders are defined as patients achieving only a moderate or no response. DAS28, disease activity score 28.

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