Fig. 7

Treatment with rhACE2 prevented Ang II-mediated renal inflammation in the ApoEKO mice. The real-time PCR (a–f) and Western blot analyses (g–i) demonstrated the renal mRNA or protein expression of inflammatory cytokines in the ApoEKO mice, including TNFα, TNFRSF1A, TNFRSF1B, IL-1β, IL-6, and IL-17A (n = 4–6). GAPDH or β-actin was used as an endogenous control. RE relative expression, rhACE2 human recombinant ACE2, Ang II angiotensin II. Please see other abbreviations in Table 3. *P < 0.05; **P < 0.01 compared with ApoEKO control group; ^# P < 0.05; ^## P < 0.01 compared with ApoEKO + Ang II group.