Combined treatment with Trabectedin and α-PD-1 mAb mounted a durable tumor-specific immunity. a Schematic regimen for combination treatment in ID8 tumor model. b Mice (6 mice per group) were transplanted i.p. with 1 × 106 ID8 cells on day 0 and treated with either single or combined Trabectedin and α-PD-1 mAb as indicated in a, and overall survival of mice was recorded. c Median survival time of tumor-bearing mice was calculated. n indicates the number of tumor-bearing mice calculated. d Long-term surviving (90 days after first tumor challenge) mice from combination group (3 mice per group) were s.c. rechallenged with ID8 or unrelated TC1 tumor cells and their overall survival was recorded. e Naïve mice challenged with ID8 or TC1 tumor cells was used as control. f Mice (6 mice per group) receiving combination treatment were also injected with an α-CD4, α-CD8, α-CD4 plus α-CD8, α-NK1.1 or control mAb with 500 μg of each mAb per mouse 1 day before and 2 days after tumor challenge followed by injection of 200 μg every 5 days thereafter for the duration of the experiments and their overall survival were recorded. Data are representative of two (f) or three (b, c) independent experiments. *P < 0.05, ***P < 0.001.