Fig. 2

Blood from control rats (no trauma, no hemorrhagic shock) was withdrawn before injection (baseline B) of labeled ¹³C-glucose (iLC) and 5, 10, 15 and 35 min after. Metabolites of glycolysis and Krebs cycles were monitored, as they have been previously shown to increase in plasma after trauma/hemorrhagic shock [14]. In left, the total levels of the metabolite (integrated peak areas—arbitrary units) are indicated through stacked bar graphs, including the unlabeled parent (blue M + 0) and heavy isotopologues (either M + 2, M + 3, M + 4 or M + 6 depending on the expected labeling pattern from catabolism of ¹³C-glucose—a schematic overview is provided in the upper right corner). In the right hand panels, only heavy isotopologues (red, yellow, orange, green) are shown. Glucose injection did not affect metabolic profiles, while progressive distribution of heavy carbon atoms was observed for all metabolites. Glycolytic products pyruvate and lactate reached the steady state after 5—15 min from spiked in injection of glucose.