Generation of lentivirus-induced dendritic cells under GMP-compliant conditions for adaptive immune reconstitution against cytomegalovirus after stem cell transplantation

Table 2 Panels for flow cytometry characterization of SmyleDCpp65

	Viability	Pan maker	Dendritic cell	Activated dendritic cell	Cell impurities	Intracellular detection of pp65 antigen
Panel I	SSC & 7AAD^–	CD45 (KromeOrange)	CD14 (APC 750) CD11c bright (PC7)	HLA-DR (Pacific Blue)/CD86 (PE) HLA-DR (Pacific Blue)/CD80 (APC)	CD3 & CD19 (APC700)	n.a.
Panel II	n.a.	CD45 (KromeOrange)	CD14 (APC750) CD11c bright (PC7)	n.a.	CD56 (PC5.5)	Anti-pp65 Ab plus secondary Ab (FITC)

Markers used in the characterization of SmyleDCpp65 after thawing and in vitro culture.
Panel I markers and fluorochromes used for detection of surface molecules in viable, non-permeabilized cells, Panel II markers and fluorochromes used for detection of surface molecules and pp65 in permeabilized cells.

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