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Table 2 Panels for flow cytometry characterization of SmyleDCpp65

From: Generation of lentivirus-induced dendritic cells under GMP-compliant conditions for adaptive immune reconstitution against cytomegalovirus after stem cell transplantation

  Viability Pan maker Dendritic cell Activated dendritic cell Cell impurities Intracellular detection of pp65 antigen
Panel I SSC & 7AAD CD45 (KromeOrange) CD14 (APC 750)
CD11c bright (PC7)
HLA-DR (Pacific Blue)/CD86 (PE)
HLA-DR (Pacific Blue)/CD80 (APC)
CD3 & CD19 (APC700) n.a.
Panel II n.a. CD45 (KromeOrange) CD14 (APC750)
CD11c bright (PC7)
n.a. CD56 (PC5.5) Anti-pp65 Ab plus secondary Ab (FITC)
  1. Markers used in the characterization of SmyleDCpp65 after thawing and in vitro culture.
  2. Panel I markers and fluorochromes used for detection of surface molecules in viable, non-permeabilized cells, Panel II markers and fluorochromes used for detection of surface molecules and pp65 in permeabilized cells.