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Table 1 Range of cell recovery at different steps of processing and after thawing (n = 3)

From: Generation of lentivirus-induced dendritic cells under GMP-compliant conditions for adaptive immune reconstitution against cytomegalovirus after stem cell transplantation

Donor

# PBMNCs in leukapheresis

# CD14+ selected (% of total leukapheresis)

# CD14+ transduced (% from selected CD14)

# Cells after transduction (% recovery from input)

# Cells cryo-preserved/mL/vial

# Cells after thawing (day 0) (% from cryopreserved)

Transduction efficiency LV copies/cell

% pp65 expression (day 7 CD45+/CD11c+)

% Activated DCs− (day 7 CD45+/CD11c+/CD80+/HLA-DR+)

#1

6.08 × 109

1.01 × 109 (17%)

1.5 × 108 (15%)

6.90 × 107 (46%)

2 × 106

0.6 × 106 (30%; n = 2)

n.a.

44.40

93.50

#2

2.07 × 1010

1.62 × 109 (8%)

1.5 × 108 (9%)

5.95 × 107 (40%)

2 × 106

1.16 × 106 (58%; n = 3)

0.66

39.40

95.50

#3

9.07 × 109

4.67 × 108 (5%)

1.5 × 108 (32%)

1.23 × 108 (82%)

2 × 106

1.73 × 106 (87%; n = 3)

1.31

44.50

97.20

Mean ± SEM

1.19 × 1010 ± 4.46 × 109

1.03 × 109 ± 3.3 × 108 (10%)

1.5 × 108 (19%)

8.4 × 107 ± 1.9 × 107 (56%)

2 × 106

1.16 × 106 ± 3.3 × 105 (58%)

0.99

42.8 ± 1.7

95.4 ± 1.1

  1. Standardized developmental runs of SmyleDCpp65 production were performed as triplicate independent runs using monocytes from three different healthy adult volunteers. Cell numbers and percent recovery after each production step are shown. Transduction efficiency and variability in the DC immune phenotype are shown.
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Journal of Translational Medicine

ISSN: 1479-5876

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