Characterization of SmyleDCpp65 after in vitro culture for 5 or 7 days without exogenous cytokines. a Average frequency of activated DCs co-expressing HLA-DR/CD80. b Average frequency of pp65+ cells detected after intracellular staining and FACS. c Concentration of transgenic cytokines GM-CSF and IFN-α in cell supernatants harvested AT, and on days 5 and 7 after thaw (pg/106 cells/mL). d Concentration of endogenously up-regulated MCP-1 and IL-8 in cell supernatants harvested AT, and on days 5 and 7 after thaw (pg/106 cells/mL). Data represent the combined data obtained for three independent SmyleDCpp65 production runs, two independent analyses for GMP1 and three independent analyses for GMP2 and GMP3 (in total 8 analyses). Bar graphs indicate mean and error bars indicate mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001.