Figure 4

Characterization of SmyleDCpp65 after in vitro culture for 5 or 7 days without exogenous cytokines. a Morphology analyses by phase microscopy. SmyleDCpp65 thawed and cultured for 3, 5 or 7 days showing high viability throughout and acquisition of typical DC morphology and cell clustering from day 5 onwards. b Methods for flow cytometry analyses for characterization of SmyleDCpp65 identity [Panel I (viable)—DC phenotype] and potency [Panel II (permeabilized)—pp65 expression] and representative examples for each GMP batch analyzed. c Average frequency of viable DCs (CD14⁻, 7AAD⁻, CD45⁺ CD11c^bright cells) for all analyses of GMP batches combined. d Average frequency of activated DCs co-expressing HLA-DR/CD86. Data represent the combined data obtained for three independent SmyleDCpp65 production runs, two independent analyses for GMP1 and three independent analyses for GMP2 and GMP3 (in total 8 analyses). Bar graphs indicate mean and error bars indicate mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001.