Figure 3

Characterization of transduced CD14⁺ monocytes after thaw (AT). a Phase microscopy of transduced CD14⁺ cells AT showing viable monocytes. b Representative example of flow cytometry gating for QC and batch release criteria, showing high viability, purity and expected monocyte characteristics of the product. c Overall viability (7AAD^neg), purity (CD45⁺CD14⁺) and quantification of residual cells (T cells and B cells: CD45⁺ CD3⁺/CD19⁺ and NK cells: CD45⁺CD56⁺) for the three SmyleDCpp65 production runs (GMP1: n = 2; GMP2: n = 3; GMP3: n = 3 independent analyses). d Detection of IDLV copies in tDNA extracted from transduced SmyleDCpp65 and cognate non-transduced monocytes (both groups AT) by RT-q-PCR. PCR signal in transduced monocytes compared with baseline is indicated. Bar graphs indicate mean and the error bars indicate mean ± SEM.