Standardized production of IDLV-G2α2pp65 under GMP compliant conditions up-scaling, recovery and titration. a Schematic representation of pilot batch of the lentiviral vector production performed under GMP compliant conditions. In process QC analyses are shown. b Infective titer of IDLV samples collected during different steps of GMP-compliant production. Indicator cells (HT1080) were transduced and the number of vector copies in genomic DNA was determined by RT-q-PCR. c Quantification of IDLV physical titer as p24 equivalent by ELISA.