The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

Table 1 Effect on antioxidant parameters in absence (CdCl₂) and presence of AEIA (AEIA + CdCl₂) and AEEF (AEEF + CdCl₂) in isolated mice hepatocytes

Parameters	Control	CdCl₂ (30 µM)	AEIA (400 µg/ml) + CdCl₂ (30 µM)	AEEF (400 µg/ml) + CdCl₂ (30 µM)
TBARS (µg/g of tissue)	3.65 ± 0.24	6.89 ± 0.54^#	4.89 ± 0.45*	5.11 ± 0.51*
Protein carbonyl (nmol/mg of protein)	41.75 ± 4.24	76.54 ± 5.33^#	52.82 ± 4.79**	54.11 ± 3.96**
CAT (U/mg of protein)	234.56 ± 14.33	168.67 ± 10.89^#	234.56 ± 14.92**	228.92 ± 12.78*
SOD (U/mg of protein)	86.54 ± 4.12	55.54 ± 3.96^#	75.44 ± 4.06**	72.12 ± 4.35*
GST (µmol/h/mg protein)	0.92 ± 0.11	0.54 ± 0.07^$	0.88 ± 0.09*	0.83 ± 0.07
GPx (nmol/min/mg of protein)	76.67 ± 4.12	51.78 ± 3.50^#	71.58 ± 4.21**	67.43 ± 3.98**
GR (nmol/min/mg of protein)	82.01 ± 4.45	54.15 ± 3.92^#	72.67 ± 4.05*	70.84 ± 4.96*
G6PD (nmol/min/mg of protein)	116.12 ± 5.56	75.60 ± 4.85^#	104.78 ± 5.11**	101.96 ± 4.76**
GSH (nmol/mg of protein)	5.23 ± 0.47	3.12 ± 0.34^#	4.81 ± 0.40*	4.67 ± 0.33*

Values are expressed as mean ± SE (n = 3). ^#Values differ significantly from normal control (p < 0.01). ^$ Values differ significantly from normal control (p < 0.05). * Values differ significantly from CdCl₂ control (p < 0.05). ** Values differ significantly from CdCl₂ control (p < 0.01). CAT unit, ‘U’ is defined as µmoles of H₂O₂ consumed per minute. SOD unit, ‘U’ is defined as the µmoles inhibition of NBT reduction per minute.

ISSN: 1479-5876