Fig. 5From: Decreased long noncoding RNA SPRY4-IT1 contributing to gastric cancer cell metastasis partly via affecting epithelial–mesenchymal transitionSPRY4-IT1 influences gastric cancer cells epithelial–mesenchymal transition. a qRT-PCR assays were performed to detect E-cadherin and Vimentin expression in BGC823 cells transfected with pCDNA-SPRY4-IT1 or si-SPRY4-IT1. b Western blot analysis of E-cadherin and Vimentin protein levels in BGC823 cells transfected with pCDNA-SPRY4-IT1 or si-SPRY4-IT1. GAPDH protein was used as an internal control. c, d Fluorescence immunohistochemistry analysis of E-cadherin and Vimentin protein levels in BGC823 cells transfected with pCDNA-SPRY4-IT1 or si-SPRY4-IT1. *P < 0.05.Back to article page