The effect of SPRY4-IT1 on gastric cancer cells invasion and metastasis. a, b Transwell assays were used to investigate the changes in migratory and invasive abilities of BGC823 and SGC7901 cells transfected with pCDNA-SPRY4-IT1 or empty vector. c, d Analysis of an experimental metastasis was performed by injecting SPRY4-IT1-overexpressing BGC823 cells into nude mice. e Visualization of the entire lungs and HE-stained lung sections. *P < 0.05 and **P < 0.01.