Fig. 2

DNMT1 suppresses SPRY4-IT1 expression in gastric cancer cells. a QRT-PCR analysis of SPRY4-IT1 expression levels in gastric cancer cell lines (SGC7901, AGS, MKN-45, MKN-28, HGC27 and BGC823) compared with normal human gastric epithelial cell line (GES-1). b qRT-PCR analyses of SPRY4-IT1 expression level following treatment of BGC823 and SGC7901 cells with 5-aza-CdR. c qRT-PCR analyses of DNMT1 or SPRY4-IT1 expression level following treatment of BGC823 and SGC7901 cells with si-DNMT1. d ChIP analyses of DNMT1 binding ability to SPRY4-IT1 promoter region, and anti-AcH3 was used as the positive control binding to GAPDH promoter region. Fold changes using the 2^−ΔΔCt method. IgG was used as a negative control. *P < 0.05 and **P < 0.01.