Fig. 3

PTEN and p21 are targets of miR-106b. a PTEN and p21 3′UTRs contain predicted miR-106b binding sites. In the figure the alignment of the seed regions of miR-106b with PTEN and p21 3′UTRs is shown. b The expression levels of PTEN and p21 after the inhibition of miR-106b via lentiviral transduction in SW480 cells or the overexpression of the same miRNA by oligonucleotide transfection or lentiviral transduction in SW620 cells were detected using western blot. c The mRNA expression levels of PTEN after the inhibition of miR-106b in SW480 cells or the overexpression of the same miRNA in SW620 cells was detected using qRT-PCR. **p<0.01. d PTEN 3′UTRs are targets of miR-106b. pluc3-PTEN that contained a wild-type or mutated PTEN 3′UTRs (indicated as WT or mut on the X-axis) was transfected into SW620 or SW480 cells. The relative repression of firefly luciferase was standardized to a transfection control. The reporter assays were performed three times with essentially identical results. **p<0.01. e p21 3′UTRs are targets of miR-106b. pluc3-p21 that contained a wild-type p21 3′UTRs was transfected into SW620 or SW480 cells. The relative repression of firefly luciferase was standardized to a transfection control. The reporter assays were performed three times with essentially identical results.**p<0.01.