Figure 3

IFN-γ production by CD4+ cells stimulated with C. Albicans before and after depletion of IL-17-secreting cells. a CD4+ cells were stimulated for 24 h with peptides from C. Albicans, then IL-17- secreting cells were then immunomagnetically depleted and negative fraction was cultured for a further 24 h in the presence of C. Albicans peptides and analyzed for intracellular IFN-γ expression. Representative dot plots of CD4+/IFN-γ+ cells from HV and AML patient before and after depletion of IL-17-secreting cells are shown. b Pooled data from 5 HV and 5 AML patients are shown as mean values and standard deviation (n.s. not significant).