IL-10 and IFN-γ intracellular production by Th17 cells in HV and AML patients. a Representative flow cytometric data of IL-17 and IL-10 double release by CD4+ T cells from HV and AML primed with IL-6 and PMA + I. b Representative dot plots of IL-17 and IFN-γ simultaneous production from a HV and an AML patient. c IL-17/IL-10 pooled data from HV (white circles) and AML patients (black circles) and mean values (bars). The frequency of CD4+ T cells that simultaneously secreted IL-17 and IL-10 was 7.5-fold increased in AML patients compared with HV. d IL-17 and IFN-γ production data derived from the entire series are shown as mean and standard deviation The concomitant intracellular production of IFN-γ and IL-17 by CD4+ cells was not significantly different from that of HV or patients (P > 0.05).