Figure 1

Alterations in T helper cells from HV and untreated AML patients. a Representative dot plots of cytokine production by CD4+ cells isolated from a HV before and after in vitro priming with IL-6 and then with phorbol 12-myristate 13-acetate (PMA) and ionomycin (I). b Representative dot plots of CD4+ cells from an AML patient before and after stimulation with IL-6 and PMA + I. c Pooled data obtained after stimulation from 30 HV (white circles) and 30 AML patients (black circles) and mean values (bars). d Gating strategy used to identify the CD4+ CD25^highFoxP3+ cells. e Mean and standard deviation of Treg frequency from HV and AML patients.