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Figure 3 | Journal of Translational Medicine

Figure 3

From: Poly(ADP-ribose) polymerase as a novel regulator of 17β-estradiol-induced cell growth through a control of the estrogen receptor/IGF-1 receptor/PDZK1 axis

Figure 3

Effect of PARP inhibition on PDZK1 mRNA in E2-stimulated MCF-7 cells. MCF-7 cells were treated with E2 for 24 h in the absence or presence of 1 or 2 μM TIQ-A. Total RNA was prepared and subjected to reverse transcription followed by conventional (a) or quantitative (b) PCR with primers specific to human PDZK1 or β-actin. Difference from untreated controls; §difference from E2-treated cells; p < 0.05. c MCF-7 cells were transduced with a viral vector encoding control shRNA or a shRNA targeting human PARP-1. Cells were then treated with E2 for 24 h after which total RNA was prepared and subjected to conventional RT-PCR with primers specific to human PDZK1 or β-actin.

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