Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus traps

Table 1 Summary of treatment conditions on BV-2 and IMA2.1 cells and their effects on VACV infection

Treatment condition	BV-2	IMA2.1	BV-2	IMA2.1
Treatment condition	Virus progeny^a		VACV-mediated cell death^b
M2 phenotype
w/o	+	0	+	−
IL-4	+	nt	+	−
M1 phenotype
IFN-γ	−	−	−	−
IFN-γ + LPS	−	−	−	−
LPS	−	−	−	−

nt not tested, w/o without stimulation.
^aVirus progeny (24 and 72 hpi) related to the infection medium was determined by standard plaque assays. [+] indicates an increase of the virus titer from the infection medium, [0] virus titer at the same level as the infection medium, [−] virus titer below the infection medium.
^bVACV-mediated cell death was related to uninfected controls (24–72 hpi) and measured by MTT cell survival assays. [+] more than 50% of cells were killed by LIVP 1.1.1 [−] less than 50% of cells were killed by LIVP 1.1.1.

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