Figure 7From: Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus trapsAnalysis of VACV infection in organotypic slice cultures with tumor cell implants. a Organotypic slice cultures (OSCs) from adult mice were established and implanted with 1 × 104 Gl261-RFP or U87-MG-RFP cells. 7 days after implantation, OSCs were infected with 5 × 106 pfu LIVP 1.1.1/well. b Standard plaque assay was performed 24 and 72 h after infection. Two-sided t test with unequal variances was used for statistics *p < 0.05. 72 h after infection OSCs implanted with GL261-RFP (c) and U87-MG-RFP (d) cells were stained with Hoechst and anti-VACV antibody to visualize infected tumor spheres. e, f IFN-γ-stimulated or unstimulated BV-2 cells were applied directly onto the OSCs implanted with GL261 cells. 24 h later OSCs were infected with LIVP 1.1.1 and stained after additional 24 h with FITC-IB4, Hoechst and anti-VACV antibody. Yellow arrows indicate exemplarily infected or non-infected BV-2 microglial cells.Back to article page