Figure 1From: Microglia and astrocytes attenuate the replication of the oncolytic vaccinia virus LIVP 1.1.1 in murine GL261 gliomas by acting as vaccinia virus trapsInjection of VACV into mouse brain tissues with and without implanted gliomas. a C57BL/6 and Foxn1nu/nu mice with (w) and without (w/o) GL261 brain tumors were infected with 5 × 106 pfu LIVP 1.1.1 (i.t). For analysis of viral titers, brains were excised 1, 3 and 7 days after virus injection and analyzed by standard plaque assay [n = 4/5 per group (w) and n = 3 per group (w/o) tumor]. All samples were measured in duplicate (data are mean values ± SD; t test *p < 0.05). b Immunohistochemical staining for astrocytes (anti-GFAP, red) and VACV (anti-VACV, green) 1 dpi in GL261 implanted tumors. Shown are representative examples.Back to article page