Figure 5

Purified CD4⁺ T cells procured from spleens of OVA-sensitized WT or PARP-1^−/− mice were stimulated with anti-CD3 and anti-CD28 antibodies and then skewed into a Th1 or Th2 phenotype in the presence or absence of 5 μΜ olaparib. RNA was extracted then used to generate corresponding cDNA followed by quantitative PCR with primer sets specific for mouse gata-3, il-4, t-bet, ifn-γ, or β-actin. Data is expressed as fold change with β-actin as a reference gene. Asterisk difference from CD3/CD28-stimulated cells; p < 0.01.