Figure 5

Vemurafenib resistant melanoma tumors are sensitive to glutaminase inhibitor treatment in vivo. a Nod Scid Gamma (NSG) mice were injected with 5 × 10⁶ of M249 parental or single drug resistant cells subcutaneously on the right flank. When tumor size reached an average of 100 mm³ tumor cell volume, treatment with 15 mg/kg of BPTES or vehicle control (DMSO) was provided every other day through intraperitoneal injection. Measurements were taken for tumor length and width. Tumor volume (mm³) was calculated by multiplying (length × width × width)/2. Graph represents mean tumor volume ± SD. b Nude mice were injected with 2 × 10⁶ of M249 resistant cells subcutaneously on right flank. When tumor size reached an average of 100 mm³ tumor cell volume, treatment with 20 mg/kg of L-DON or vehicle control (water) was provided twice a week through intraperitoneal injection. Graph represents mean tumor volume ± SD.