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Figure 3 | Journal of Translational Medicine

Figure 3

From: Vemurafenib resistance reprograms melanoma cells towards glutamine dependence

Figure 3

Vemurafenib resistant cells are more sensitive to glutaminase inhibitors. a M249 and M229 parental single drug resistant (SDR) cells were cultured in the presence of medium control (DMSO) or 10 μM BPTES for 48 h. SDR cell lines were cultured in combination of vemurafenib (1 μM) and BPTES. Surviving cell fraction percentage (survival %) compared to vehicle control (DMSO) was assessed using DIMSCAN technology (n = 6, ***p < 0.001, *p < 0.05). b M249 and M229 parental and single drug resistant cells were cultured in the presence of vehicle control (water) or 10 μM L-DON for 48 h. SDR cell lines received a combination of vemurafenib (1 μM) and L-DON. Surviving cell fraction percentage (survival %) compared to vehicle control (DMSO) was assessed using DIMSCAN technology (n = 6, ***p < 0.001, *p < 0.05). c M249 parental and double drug resistant (DDR) cells were cultured in the presence of medium control (DMSO) or 10 μM BPTES for 48 h. DDR cell lines were cultured in combination with vemurafenib (1 μM), selumetinib (1 μM), and BPTES. Surviving cell fraction percentage compared to vehicle control (DMSO) (survival %) was assessed using DIMSCAN technology (n = 6, ***p < 0.001). d M249 single (SDR) and double drug (DDR) resistant lines were cultured in the presence or absence of inhibitor (1 μM vemurafenib for SDR and 1 μM vemurafenib and 1 μM selumentib for DDR) and subsequently treated with 10 μM BPTES or deprived of glutamine (Gln) for 48 h. Surviving cell fraction percentage compared to vehicle control (DMSO) (survival %) was assessed using DIMSCAN technology.

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