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Figure 2 | Journal of Translational Medicine

Figure 2

From: Vemurafenib resistance reprograms melanoma cells towards glutamine dependence

Figure 2

Vemurafenib resistant cells are more sensitive to glutamine deprivation. a Melanoma M249 and b M229 single drug resistant (SDR) cells were cultured in the presence of medium with or without glutamine (Gln) or glucose (Gluc). After 24 h representative images and surviving fractions (survival %) compared to control cells in complete medium are shown using DIMSCAN, a microcomputer fluorescence-based cytotoxicity assay (n = 6, **p < 0.01). Western blot analysis was also used to assess levels of cleaved PARP as an indicator of apoptosis in both c M249 and d M229 parental and SDR cell lines. e Apoptosis was also assessed using flow cytometry of M249 parental and resistance cells stained with AnnexinV and DAPI. M249 parental and SDR cells were cultured in the presence of medium with and without glutamine for 24 h. Representative dot blots and percentages of AnnexinV positive cells are shown. f Melanoma double drug resistant (DDR) cell line M249 DDR5 was cultured in the presence of medium with and without glutamine (Gln) or glucose (Gluc). After 24 h representative images and surviving fractions (survival %) compared to control cells in complete medium are shown using DIMSCAN, a microcomputer fluorescence-based cytotoxicity assay (n = 6, ***p < 0.001). g Apoptosis was also assessed using flow cytometry of M249 parental and DDR cells stained with AnnexinV and DAPI. M249 parental and DDR cells were cultured in the presence of medium with and without glutamine for 24 h. Representative dot blots and percentages of AnnexinV positive cells are shown.

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