Histological evaluation of proliferative cellular nuclear antigen (PCNA)-positive nuclei at 11 and 21 days after different MSC treatments. Panels
1, 2 autologous ASC-treated wounds show a high number of PCNA-positive nuclei in epidermal layers. Panels
3, 4 allogeneic ASC wounds display several PCNA-positive cells. Panels
5, 6 in wounds treated with BM-MSCs the number of PCNA-positive cells is low. Panels
7, 8 saline-treated wounds show no epithelial cells marked with PCNA, but a considerable immunopositivity for fibroblasts and endothelial cells was detected. Eschar (asterisk). Immunostaining of PCNA. Scale bar 200 μm; original mag. ×100.